Cell Growth Kinetics Ppt

Specific growth rate has dimensions T -1. Growth Kinetics Introduction - Autocatalytic reaction.

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Initial tumor diameter 05 cm.

Cell growth kinetics ppt. Most of the continuous cell lines are aneuploid possessing chromosome number between diploid and tetraploid value. Growth Kinetics of batch fermentation Introduction-Autocatalytic reaction. Cell mass concentration gL.

Increased number of biomass. Kinetics of bacterial growth is one way to determine the speed of cell biomass production and the inﬂuence of the en-vironment nutrient restricted on the speed of cell growth Schaechter2009. Where r X is the volumetric rate of biomass production with units of for example kg m -3 s -I x is viable cell concentration with units of for example kg m -3 and μ is the specific growth rate.

In the normal cells the normal p 53 gene is responsible for the arrest of cell cycle. Batch Growth Kinetics Exponential growth phase In this phase the cells have adjusted to their new environment and multiply rapidly exponentially Balanced growth all components of a cell grow at the same rate. Net specific growth rate 1time.

Growth kinetics deals with the rate of cell growth. Reactive intermediates and steady state approximation in reaction mechanisms. µ µm S K S S 30 David R.

During the log phase cell numbers increase exponentially at a constant maximum rate. V 43π r3 100 days later the tumor had grown to 4 cm. 1 the lag phase which occurs immediately after inoculation and persists until the cells have acclimated to their new environment 2 exponential growth phase during which time cell growth proceeds at an exponential rate indicated by a straight line on the semi-log plot 3 a deceleration.

The growth rate is based on the number of cells and also on the biomass concentration. Introduction Growth kinetics. For each phase of the cell cycle the mean duration and the limits of the 95 confi- dence interval are given for human tumors.

By plotting the log of viable cell concentration X with time five distinct phases of the growth curve can be identified. The phases of cell growth in. The aim of this study was.

µ specific cell growth rate hr-1 µ m maximum specific cell growth rate hr-1 S substrate concentration gL K S Saturation constant gL S when µ 12 µ m. Substrate uptake and product formation in microbial growth. GROWTH KINETICS IN CELL CULTURE The standard growth kinetics of a culture cycle begins with a lag phase proceeding through the logarithmic or exponential phase to a stationary phase and finally to the decline and death of cells.

Three factors determine tumor growth Cell cycle time of proliferating cells Growth fraction Cell loss fraction Cell cycle times for tumor cells TC 1-5 days Tumor volume doubling times TD 40-100 days Example. Cell Growth Kinetics Introduction Growth patterns and kinetics in batch culture - growth phases - effect of factors. Cell Growth Kinetics Introduction Growth patterns and kinetics in batch culture - growth phases - effect of factors.

Reaction mechanisms and rate laws. The study of chemical kinetics concerns the second and third questionsthat is the rate at which a reaction yields products and the molecular-scale means by which a reaction occurs. Rate of cell growth is described by the equation.

DXdt rate of growth in reactor rate o loss from reactorμX-Dx Under steady state conditions the rate of growth rate of. The inoculated microorganism will pass through a number of growth phases Fig. CONTINUOUS GROWTH KINETICS The actual growth rate depends not only on the volumetric flow rate of the medium into the reactor but also on the dilution rateD D FV The net change in the cell concentration over a period of time may be expressed as.

Increased number of biomass. If you inoculate unicellular microorganisms into a sterilized medium and measure the cell density with respect to time there are 6 phases. Growth rate is independent of nutrient concentration as nutrients are in excess.

Oxygen supply - heat generation PowerPoint PPT presentation free to view Thermodynamics and kinetics - Heats of reaction Thermodynamic laws Electrochemistry Kinetics Acid-Base Equilibrium calculations Heats of Reaction 1 cal. R X μx. Shonnard Michigan Technological University Batch Culture Growth Model.

The rate of growth is directly related to cell concentration substrates cells extracellular products more cells S X P nX S. In mathematical terms we can write. Enzyme kinetics Michaelis-Menten rate form.

Cell mass concentration gL. Stoichiometry and Kinetics of Cell Growth and Product Formation BASIC TERMINOLOGY Anchorage-dependent Attachment to a suitable substrate is required for. The rate of growth is directly related to cell concentration substrates cells extracellular products more cells S X P nX S.

Kinetics of bacterial growth is inﬂuen-ced by environmental factors such as temperature pH and NaCl Sutherland et al1994. 252 GROWTH KINETICS IN BATCH CULTURE Batch culture occurs in a closed system that contains an initial limited amount of substrate. Kinetics of cell growth and enzymes.

This is the most common kinetic model for cell growth. Most often the deletion or mutation of the p 53 gene is responsible for continuous proliferation of cells. KINETICS OF TUMOR GROWTH 115 DEATH DlFFERENTIATlON t INHIBITOR FACTOR ui iynthesir DNA Slis 17h 10 to 730 h Fig.

In this module we will examine the factors that influence the rates of chemical reactions the mechanisms by which reactions proceed and the quantitative techniques used to determine and describe the rate at which reactions. The ability of the cells to grow continuously in cell lines represents genetic variation in the cells.

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